›› 2010, Vol. 41 ›› Issue (1): 65-69.doi: 10.3969/j.issn.0529-1356.2010.01.013

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Clinical significance of thrombospondin 4 expression in gastric carcinoma

  

  1. 1.Key Laboratory of Infection and Oncology, Research Center of Molecular Medicine, Fujian Medical University,Fuzhou350014,China;2.Department of Pathology, Fujian Provincial Tumor Hosptal, the Teaching Hospital of Fujian Medical University,Fuzhou350014, China
  • Received:2009-08-03 Revised:2009-10-17 Online:2010-02-06
  • Contact: LIN Jian-Yin

Abstract: Objective The aim of this study was to measure quantitatively the TSP-4 mRNA expression and its significance in gastric carcinoma was evaluated by correlating its expression with clinicopathological features, microvessel density(MVD) and MMP-9.Methods Eighty-two patients with gastric carcinoma were recruited in this study. TSP-4 mRNA expression in gastric carcinoma and adjacent tissue was detected by real-time PCR. CD34 and MMP-9 protein expression were examined by immunohistochemistry. MVD was determined according to CD34-positive tubular structures. Results The expression level of TSP-4mRNA in gastric carcinoma was obviously higher than those of adjacent tissue(EM>P/EM>=0.03) and it was associated with tumor size, histological type, lymph node metastasis, MVD and MMP-9(EM>P/EM>=0.002, P=0.031, EM>P/EM>=0.014,EM>r/EM>=0.67 EM>P/EM><0.01, EM>P/EM>=0.008),but not sex, age and depth of invasion. (EM>P/EM>=0.53,EM>P/EM>=0.57,EM>P/EM>=0.15).Conclusion TSP-4 may play an important role in occurrence and progression of gastric carcinoma and that the effect of TSP-4 on tumor growth and metastasis may be exerted through reg

Key words: Gastric carcinoma, Thrombospondin-4, Microvessel density, Matrix metalloproteinase-9, Immunohistochemistry, RT-PCR, Human

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